APOLIPOPROTEIN FINGERPRINTING TECHNIQUE

A method for determining the concentration and modifications of apolipopr otein in biological samples including plasma, serum, and lipoprotein fractio ns, by obtaining a sample from a patient, adding a specific volume of an int ernal standard to the sample, applying the sample to a surface-enhanced, Pro tein G-coated, antibody-bound chip and removing unbound sample components, a nalyzing the sample by mass spectrometry, determining the concentration of t he apolipoprotein using values of internal standards, and evaluating the con centration of the apolipoprotein, its isoforms, amino acid substitutions and modifications for use as a tool for diagnosing diabetes, stroke, stress, Al zheimer's disease, and cardiovascular diseases (for example, lipid disorders , metabolic syndrome, obesity, atherosclerosis, etcetera).