METHOD FOR THE RANDOM DIVERSIFICATION OF A GENETIC SEQUENCE WHILE PRESERVING THE IDENTITY OF SOME INNER SEGMENTS OF SAID GENETIC SEQUENCE

The invention relates to a very general method for the random diversification of a nucleotide sequence S by PCR while preserving the identity of some domains of said sequence S; the invention also relates to a bank of nucleotide sequence thus diversified, and to diversified proteins obtained by the...

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1. Verfasser:	IRIS, FRANCOIS
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a very general method for the random diversification of a nucleotide sequence S by PCR while preserving the identity of some domains of said sequence S; the invention also relates to a bank of nucleotide sequence thus diversified, and to diversified proteins obtained by the expressions of the nucleotide sequences in an appropriate host. La présente invention vise un procédé très général pour diversifier de manière aléatoire une séquence nucléotidique S par PCR, tout en préservant l'identité de certains domaines de ladite séquence S, une banque de séquences nucléotidiques ainsi diversifiées, les protéines diversifiées obtenues par expressions de ces séquences nucléotidiques dans un hôte approprié.