COMBINATION HEPATITIS C VIRUS ANTIGEN AND ANTIBODY DETECTIONMETHOD

An in vitro method that allows detection of hepatitis C by detecting hepa titis C virus (HCV) core protein and antibodies to HCV core protein (anti-co re antibodies) in a single assay is provided. Cross-reactivity is eliminated in the method preferably by utilizing short peptides, each of which has...

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Bibliographische Detailangaben
Hauptverfasser:	RODGERS, BRIAN C, BURCH, GRAHAM J
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY ORGANIC CHEMISTRY PEPTIDES PHYSICS TESTING
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Beschreibung
Zusammenfassung:	An in vitro method that allows detection of hepatitis C by detecting hepa titis C virus (HCV) core protein and antibodies to HCV core protein (anti-co re antibodies) in a single assay is provided. Cross-reactivity is eliminated in the method preferably by utilizing short peptides, each of which has an amino acid sequence that corresponds to an immunodominant region of the nati ve core protein but which does not wholly encompass the epitope bound by the antibodies utilized in the method. The method can be used to detect the pre sence of HCV in a subject, and/or to determine the suitability of donor bloo d or blood products for transfusion purposes. Also provided are diagnostic k its for carrying out the method and a process for selecting suitable capture peptides and monoclonal antibodies for use in the combination method.