MODIFICATION OF XYLANASES TO INCREASE THERMOPHILICITY, THERMOSTABILITY AND ALKALOPHILICITY

A modified Family 11 xylanase enzyme comprising cysteine residues at positions 99 and 118 to form an intramolecular disulfide bond is provided. The modified xylanase is produced by substitution of an amino acid at position 99, 118 or both positions 99 and 118 with a cysteine to produce the intramole...

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Bibliographische Detailangaben
1. Verfasser:	SUNG, WING L
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	APPARATUS THEREFOR BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMES ENZYMOLOGY IMPREGNATING OR COATING OF PAPER MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PAPER NOT OTHERWISE PROVIDED FOR PAPER-MAKING PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D PROCESSES OF PREPARING SUCH COMPOSITIONS PRODUCTION OF CELLULOSE PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PULP COMPOSITIONS REGENERATION OF PULPING LIQUORS SPIRITS TEXTILES TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G VINEGAR WINE
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Beschreibung
Zusammenfassung:	A modified Family 11 xylanase enzyme comprising cysteine residues at positions 99 and 118 to form an intramolecular disulfide bond is provided. The modified xylanase is produced by substitution of an amino acid at position 99, 118 or both positions 99 and 118 with a cysteine to produce the intramolecular disulfide bond. Xylanases of the invention display improved thermophilicity, alkalophilicity or thermostability relative to wild-type xylanases. Such xylanases find use in a variety of applications in industry that require enzyme activities at temperatures and/or pH values above that of the native enzyme.