METHOD AND BIOREACTOR FOR GENERATING EX VIVO TISSUE MATRIX

The present invention relates to methods of generating an ex vivo tissue-like system in a bioreactor system and a bioreactor capable of performing the method. The bioreactor maintains a culture of either homologous or heterogenous cells in an organized arrangement in three-dimensions and allows the development of differing microenvironments conducive to tissue expansion and maintenance for extended periods of time with the ability to change the microenvironment. The bioreactor provides a matrix material configured to receive cells in a first fluid, a first chamber configured to flow the first fluid including the cells to the matrix material, a first gas permeable membrane configured to permit a first gas to flow therethrough to the first fluid in the first chamber; and a gassing chamber operably linked to the first chamber. The device and method may be used for ex vivo study of tissues and tissue organization. La présente invention concerne des procédés de génération d'un système de type tissulaire ex vivo dans un système de bioréacteur capable de maintenir une production continue et une sortie de cellules et de tissus, ainsi qu'un système tissulaire ex vivo ainsi obtenu.