METHODS FOR THE DETERMINATION OF ANTIBODY IGG AVIDITY, BY COMPARING LIQUID TO SOLID PHASE AVIDITY

The present invention relates to methods of determining anti-infectious agent lgG avidity, for example, human anti-cytomegalovirus and human anti-toxoplasma lgG, using a competitive assay format. The methods include the steps of adding a purified antigen from the infectious agent to a solution to create a liquid phase antigen, and coating the purified antigen onto a solid phase to create a solid phase antigen; assaying separately the liquid and solid phase antigens with test samples from the patient and determining the formation of antibody/antigen complexes; and determining the ratio of anti-infectious agent antibody present in the test sample when assayed by the liquid phase antigen compared to the solid phase antigen, wherein the ratio is proportional to the level of antibody present in the patient. La présente invention concerne des procédés de détermination d'avidité pour un IgG anti-agent infectieux, par exemple un IgG anti-cytomégalovirus et anti-toxoplasme humain, utilisant un format de dosage compétitif.