FLUOROGENIC MEASURMENT OF THROMBIN ACTIVITY IN A THIN LAYER OF WHOLE BLOOD

The present invention relates to a method for in vitro determining the time course of thrombin activity and to means enabling the measurement of active thrombin following its generation in a sample in vitro. The present invention also relates to the use of this method for the detection or the monito...

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Bibliographische Detailangaben
Hauptverfasser:	GIESEN, PETER, NIJHUIS, SEBASTIAAN, HEMKER, HENDRIK COENRAAD, BEGUIN, SUZETTE, WAGENVOORD, ROBERT, AL-DIERI, RAED
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method for in vitro determining the time course of thrombin activity and to means enabling the measurement of active thrombin following its generation in a sample in vitro. The present invention also relates to the use of this method for the detection or the monitoring of the condition of a patient, including the detection or monitoring of a pathological condition related to blood coagulation deficiency. The method comprises contacting a layer of a blood sample with a fluorogenic substrate of thrombin, allowing thrombin to generate in said sample; measuring the fluorescence emitted from the surface of the layer by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on the fluorogenic substrate.