METHODS OF REFOLDING MAMMALIAN GLYCOSYLTRANSFERASES

The present invention provides methods of refolding mammalian glycosyltransferases that have been produced in bacterial cells, and methods to use such refolded glycosyltransferases, including glycosyltransferase mutants that have enhanced ability to be refolded. The invention also provides methods o...

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Bibliographische Detailangaben
Hauptverfasser:	SARIBAS, SAMI, HAKES, DAVID, BEZILA, DANIEL JAMES, WILLETT, SCOTT, JOHNSON, KARL F, DEFREES, SHAWN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides methods of refolding mammalian glycosyltransferases that have been produced in bacterial cells, and methods to use such refolded glycosyltransferases, including glycosyltransferase mutants that have enhanced ability to be refolded. The invention also provides methods of refolding more than one glycosyltransferase in a single vessel, methods to use such refolded glycosyltransferases, and reaction mixtures comprising the refolded glycosyltransferases. La présente invention a trait à des procédés de repliement de glycosyltransférases mammaliennes qui ont été produites dans des cellules bactériennes, et à des procédés d'utilisation desdites glycosyltransférases repliées, comprenant des mutants de glycosyltransférases qui présente une capacité améliorée de repliement. L'invention a également trait à des procédés de repliement de plusieurs glycosyltransférases dans un unique réceptacle, à des procédés d'utilisation desdites glycosyltransférases repliées, et à des mélanges réactionnels comportant les glycosyltransférases repliées.