FLUORESCENT PROBES FOR USE IN PROTEIN KINASE INHIBITOR BINDING ASSAY

The invention provides methods relating to a novel screening assay format that can be applied to broad members of the protein kinase gene family. The assay uses a series of labeled, active site probes described herein that can be displaced by an inhibitor agent. The Kd for the inhibitor compound is...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	JAKES, SCOTT, BROWN, MARTHA PRISCILLA, JONES, JESSI WILDESON, LABADIA, MARK E, PROKOPOWICZ, ANTHONY S
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	ADHESIVES BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DYES ENZYMOLOGY HETEROCYCLIC COMPOUNDS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LAKES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MORDANTS MUTATION OR GENETIC ENGINEERING NATURAL RESINS ORGANIC CHEMISTRY ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES PAINTS PHYSICS POLISHES PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention provides methods relating to a novel screening assay format that can be applied to broad members of the protein kinase gene family. The assay uses a series of labeled, active site probes described herein that can be displaced by an inhibitor agent. The Kd for the inhibitor compound is derived based on the Kd of the probe for the kinase and the dose response of the inhibitor agent. The invention also provides novel active site probes suitable for use with the screening method. L'invention concerne des procédés relatifs à un format de test d'analyse pouvant être appliqué à une large gamme de membres de la famille des gènes de protéine kinase. On utilise une série de sondes de site actif marquées, décrites dans l'invention, pouvant être déplacées par un agent inhibiteur. Le K¿d? pour le composé inhibiteur est dérivé sur la base du K¿d? de la sonde pour la kinase et de la dose-réponse de l'agent inhibiteur. L'invention concerne également des sondes de site actif se prêtant à une utilisation dans le cadre du procédé considéré.