FUNGUS STRAIN AND A METHOD OF OBTAINING MANNITOL FROM THE SAME

The present invention relates to a plant mangrove-associated fungus Culvular ia lunata of International deposition no. MTCC 5109, and a simple and efficient method of obtaining high yield of pure mannitol from a plant mangrove- associated fungus Curvularia lunata, said method comprising steps of cut...

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Bibliographische Detailangaben
Hauptverfasser:	NAIK, CHANDRAKANT GOVIND, RODRIGUES, ELY, PEKETI, ASHA, WAHIDULLA, SOLIMABI, DEVI, PRABHA, D'SOUZA, LISETTE
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a plant mangrove-associated fungus Culvular ia lunata of International deposition no. MTCC 5109, and a simple and efficient method of obtaining high yield of pure mannitol from a plant mangrove- associated fungus Curvularia lunata, said method comprising steps of cutting the leaves into small pieces and placing it on potato dextrose agar (PDA) plates for 48 hours, maintaining the culture at temperature ranging between 26- 470C and salinity ranging between 4-32 ppt with occasional stress conditions for about 17-19 days to obtain mycelial mat, sonicating the mat to lyse the cells, extracting crude from the sonicated mat using methanol repeatedly, concentrating the crude, treating the concentrated crude extract with solven ts of increasing polarity, obtaining aqueous fraction as white powder residue after the treatment containing crude mannitol, purifying the crude mannitol by chromatography to obtain pure mannitol with about 75% of the total crude extract.