PECTATE LYASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM

The invention is directed to polypeptides having pectate lyase (pectinase) activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as pectate lyases to catalyze the beta-elimination or...

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Bibliographische Detailangaben
Hauptverfasser:	GERENDASH, JOEL, SOLBAK, ARNE, DAHOD, SAMUN, GRAY, KEVIN, PUROHIT, SHALAKA, JANSSEN, GISELLE, KEROVUO, JANNE, MCCANN, RYAN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CALCULATING CANDLES CARBONISING RAGS TO RECOVER ANIMAL FIBRES CHEMICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIALTO OBTAIN FILAMENTS OR FIBRES FOR SPINNING CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF COMPUTING CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES COUNTING CULTURE MEDIA DERIVATIVES THEREOF DETERGENT COMPOSITIONS DETERGENTS EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKINGOILS ELECTRIC DIGITAL DATA PROCESSING ENZYMOLOGY ESSENTIAL OILS FATTY ACIDS THEREFROM FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FODDER FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J FORESTRY HUMAN NECESSITIES HUNTING HYGIENE IMPREGNATING OR COATING OF PAPER INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NATURAL OR ARTIFICIAL THREADS OR FIBRES NEW PLANTS OR PROCESSES FOR OBTAINING THEM NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PAPER NOT OTHERWISE PROVIDED FOR PAPER-MAKING PEPTIDES PERFUMES PEST REPELLANTS OR ATTRACTANTS PHYSICS PLANT GROWTH REGULATORS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS PRODUCTION OF CELLULOSE PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PULP COMPOSITIONS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPINNING SPIRITS SUGARS TESTING TEXTILES THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES TRAPPING TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention is directed to polypeptides having pectate lyase (pectinase) activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as pectate lyases to catalyze the beta-elimination or hydrolysis of pectin and/or polygalacturonic acid, such as 1,4-linked alpha-D-galacturonic acid. The invention provides methods of treating fibers, fabrics or any pectate- or polygalacturonic acid-comprising material using one or more pectate lyases of the invention. La présente invention concerne des polypeptides possédant une activité de pectate lyase (pectinase), des polynucléotides codant lesdits polypeptides, et des procédés de fabrication et d'utilisation des polynucléotides et polypeptides précités. Les polypeptides de l'invention peuvent être utilisés comme pectate lyases pour catalyser la bêta-élimination ou l'hydrolyse de la pectine et/ou de l'acide polygalacturonique, tel que l'acide alpha-D-galacturonique lié en 1,4. L'invention concerne des procédés permettant de traiter des fibres, des tissus ou un quelconque matériau comprenant du pectate ou de l'acide polygalacturonique à l'aide d'une ou plusieurs pectate lyases de l'invention.