METHOD OF PRODUCING MICROBIAL TRANSGLUTAMINASE

METHOD OF PRODUCING MICROBIAL TRANSGLUTAMINASE

The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism i...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: YOKOYAMA, KEIICHI, KIKUCHI, YOSHIMI, ONISHI, NORIMASA, UMEZAWA, YUKIKO, DATE, MASAYO
Format: Patent
Sprache:eng ; fre
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase. L'invention concerne une métalloprotéase neutre issue d'actinomyces, permettant de couper le fragment pro-structural d'une protransglutaminase issue d'un micro-organisme, et un gène la codant. Une transglutaminase issue d'un micro-organisme activée, dont le fragment pro-structural a été excisé, peut être produite selon un procédé consistant à cultiver un micro-organisme à l'intérieur duquel le gène codant pour ladite métalloprotéase neutre issue d'actinomyces a été transféré, ce qui permet au micro-organisme de produire la métalloprotéase neutre issue d'actinomyces, puis à traiter la protransglutaminase issue du microorganisme avec celle-ci.