GENES FROM PROPIONIBACTERIUM FREUDENREICHII ENCODING ENZYMESINVOLVED IN VITAMIN B12 BIOSYNTHESIS

Four new genes, and the enzymes that they encode, are disclosed, which are present in Propionibacterium freudenreichii, and which are involved in at least five steps in the biosynthetic pathway of vitamin B12. The four enzyme s are: A: cobyrinic acid a, c-diamide synthase; B: a bifunctional enzyme,...

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Bibliographische Detailangaben
Hauptverfasser:	PEL, HERMAN JAN, HOPPER, SYLVIA
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Four new genes, and the enzymes that they encode, are disclosed, which are present in Propionibacterium freudenreichii, and which are involved in at least five steps in the biosynthetic pathway of vitamin B12. The four enzyme s are: A: cobyrinic acid a, c-diamide synthase; B: a bifunctional enzyme, whic h is a cobinamide kinase and a cobinamide phosphate guanyl transferase; C: a cobalamin 5-phosphate synthase; and D: an adenosyl transferase. Genes encodi ng the four enzymes can be placed in Propionibacteria shuttle vectors and used to transform Propionibacteria hosts, in order to improve the production of vitamin B12 or a precursor thereof on an industrial scale during fermentatio n.