A METHOD OF ELECTROPHORESIS

The present invention relates a method of electrophoretic separation of protein and/or peptide components of a sample in a convection stabilised medium. More specifically, the method comprises the steps to contact the sample with the separation medium; to apply a voltage across said medium; and to o...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	OLSSON, INGMAR, BJELLQUIST, BENGT, PALMGREN, RONNIE
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLIDMATERIALS OR FLUIDS MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROMSOLID MATERIALS OR FLUIDS MEASURING METALLURGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS SEPARATION SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS TESTING TRANSPORTING
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Beschreibung
Zusammenfassung:	The present invention relates a method of electrophoretic separation of protein and/or peptide components of a sample in a convection stabilised medium. More specifically, the method comprises the steps to contact the sample with the separation medium; to apply a voltage across said medium; and to observe the results by analysis of one or more sections of the separation medium. In the present method, a disulphide-comprising compound is added before or during the procedure to make an excess of reactive disulphide groups accessible to react with the cysteine groups of the proteins and/or peptides all through the separation procedure. The present invention also relates to electrophoretic separation media that comprises re-active disulphide groups, such as polyacrylamide gels, and the use of a solution that comprises reactive disulphide groups to pretreat an electrophoretic separation medium.