PROCESS FOR PURIFYING CHEMICALLY SYNTHESIZED RNA

Disclosed is a process for purifying chemically synthesized RNA having one or more chemical modifications, comprising: (a) loading the RNA onto an anion exchange high-performance liquid chromatography (HPLC) column; (b) eluting the RNA by passing a suitable buffer through the column to obtain an elu...

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Hauptverfasser:	DIRENZO, ANTHONY, SWEEDLER, DAVID, SULLIVAN, SEAN M, CHOWRIRA, BHARAT, MATULIC-ADAMIC, JASENKA, BEIGELMAN, LEONID, PAVCO, PAMELA, USMAN, NASSIM, WOOLF, TOD, DUDYCZ, LECH W, WINCOTT, FRANCINE E, MODAK, ANIL, MCSWIGGEN, JAMES A, TRACZ, DANUTA, STINCHCOMB, DAN T, DRAPER, KENNETH G, GRIMM, SUSAN, KISICH, KEVIN, THOMPSON, JAMES D, KARPEISKY, ALEXANDER
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed is a process for purifying chemically synthesized RNA having one or more chemical modifications, comprising: (a) loading the RNA onto an anion exchange high-performance liquid chromatography (HPLC) column; (b) eluting the RNA by passing a suitable buffer through the column to obtain an eluate; and (c) collecting the eluate from the column and recovering the RNA from the eluate, under conditions which allow for purification of the RNA. The RNA to be purified may be produced by deprotecting one or more protecting groups.