ENZYMATIC METHOD OF PRODUCING 4-0-B-D GALACTOPYRANOSYL-D-XYLOSE, 4-0-B-D-GALACTOPYRANOSYL-D-XYLOSE OBTAINED USING SAID METHOD, COMPOSITIONS CONTAINING SAME AND THE USE THEREOF IN EVALUATING INTESTINAL LACTASE

The invention relates to an enzymatic method of producing 4-0-.beta.-D- galactopyranosyl-D-xylose which is used for the in vivo evaluation of intestinal lactase activity in humans. The inventive method consists in: a) reacting D-xilose with a .beta.-D-galactopyranoside for 2 48 h in a medium buffere...

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Bibliographische Detailangaben
Hauptverfasser:	CORRALES MORALES, GUILLERMO, CANADA VICINAY, FRANCISCO JAVIER, FERNANDEZ-MAYORALAS ALVAREZ, ALFONSO, MARTIN LOMAS, MANUEL, ARAGON REYES, JUAN JOSE
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SEPARATION SPIRITS SUGARS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to an enzymatic method of producing 4-0-.beta.-D- galactopyranosyl-D-xylose which is used for the in vivo evaluation of intestinal lactase activity in humans. The inventive method consists in: a) reacting D-xilose with a .beta.-D-galactopyranoside for 2 48 h in a medium buffered to pH 5 9 and at a temperature that varies between the freezing of the mixture and 45 ~C, and subsequently adding 10-10,000 units of .beta.-D- galactosidase per gram of .beta.-D-galactopyranoside; b) stopping the reacti on by deactivating the enzyme; and c) isolating and crystallising the fractions that contain 4-O-.beta.-D-galactopyranosyl-D-xilose in a crystallisation mixture selected between acetone/methanol (5:1-20:1) and acetone/water (5:1- 20:1).