ASSAY FOR PREDICTING CELL ACTIVITY

The invention relates to a method for determining in vitro the capacity of a cell population to induce bone formation in vivo comprising the steps of: a) providing a sample of a cell population; b) dividing said sample into a firs t and a second part containing an equal number of cells; c) culturing...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	VAN BLITTERSWIJK, CLEMENS ANTONI, DA SILVA MADUREIRA MENDES, SANDRA CLAUDIA, DE BRUIJN, JOOST DICK
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for determining in vitro the capacity of a cell population to induce bone formation in vivo comprising the steps of: a) providing a sample of a cell population; b) dividing said sample into a firs t and a second part containing an equal number of cells; c) culturing the firs t part in the presence of an osteogenic stimulation factor; d) culturing the second part in the absence of an osteogenic stimulation factor; e) determini ng degrees of expression of a bone-specific protein; and f) comparing the degre es of expression of the bone-specific protein of the first part and the second part thereby providing a measure for the capacity of the bone cell populatio n to induce bone formation in vivo.