A CRYOPRESERVATION BAG ASSEMBLY FOR MAMMALIAN CELL LINES

The invention is a novel cell freezing and storage bag assembly and method f or using the assembly in the seed train expansion of mammalian cells. The bag i s constructed principally of polytetrafluoroethylene fabric. The bag is design ed to hold enough cells that a bioreactor can be inoculated dire...

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Bibliographische Detailangaben
Hauptverfasser:	WANG, DE QIAN, GARDNER, BRUCE, MERED, MOKHTAR, HEIDMANN, RUDIGER
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BABY COMFORTERS BEER BIOCHEMISTRY CHEMISTRY CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICALPURPOSES DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY DEVICES FOR RECEIVING SPITTLE DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGINGPHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL ORADMINISTERING FORMS ENZYMOLOGY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention is a novel cell freezing and storage bag assembly and method f or using the assembly in the seed train expansion of mammalian cells. The bag i s constructed principally of polytetrafluoroethylene fabric. The bag is design ed to hold enough cells that a bioreactor can be inoculated directly. The bag i s designed to be filled to a fraction of its maximum capacity so that the cell suspension has a very thin cross-section (less than about 10 millimeters). T he bag design includes a transfer set that can be sterilely welded to the sourc e of the mammalian cells. This sterile-weldable transfer set allows the bags t o be filled quickly with minimal risk of contamination. In the method, once ea ch bag is filled, it is sealed below the connection with the transfer set and t he bag is cut "above" the new seal (on the same side of the seal as the transfe r set). When a bioreactor is to be inoculated, the contents of the bag are drained via a sterile-weldable inoculation line. During freezing and storage , the inoculation line is protected from mechanical damage.