SUPERSECRETABLE PEPTIDES, PROCESSES FOR THEIR PRODUCTION, AND PARALLEL IMPROVEMENT OF THE SECRETED FORM OF ONE OR MORE OTHER POLYPEPTIDES

The invention relates to an expression cassette of the form Px-Sx-Bn-(ZR)-transport peptide-(Z1Z2)-protein(Y)-(Z1Z2)-protein (Ym)-T; wherein the expression cassette codes for a transport peptide linked via a sequence Z1Z2 to a second protein which in turn is linked via Z1Z2 to a protein Y1 which either corresponds to Y or can be different from Y and the transport peptide improves the rate of secretion and Y and/or Ym where Px is any promoter DNA sequence selected in such a way that optimal yields of the protein of interest become obtainable; Sx is any DNA which, accordingly, encodes any signal or leader sequence which allows optimal yields; Bn is 1-15 genetically encodable amino acids or a chemical bond; Z is the codon of an amino acid selected from the group comprising Lys and Arg; Z1 is the codon of an amino acid selected from the group comprising Lys and Arg; Z2 is the codon of an amino acid selected from the group comprising Lys and Arg; protein Ym is a DNA sequence encoding any protein which can be produced and secreted by yeast (m=1-5) or is a chemical bon (m=0); R is an argine codon; transport peptide is a DNA sequence encoding a peptide which can be transported efficiently and which can pass membranes; protein Y is a DNA sequence encoding any protein which can be produced and secreted by yeast and whose biological activity, when Ym is not a chemical bond, is not impaired by a basic dipeptide extension or allows degradation of the extension by carboxypeptidases; T is an untranslated DNA sequence advantageous to the expression, and to processes for the production and use thereof. La présente invention concerne une cassette d'expression de la forme Px-Sx-Bn-(ZR)-peptide de transport-(Z¿1?Z¿2?)-protéine (Y)-(Z¿1?Z¿2?)-protéine (Y¿m?)-T, la cassette d'expression codant pour un peptide de transport lié via une séquence Z¿1?Z¿2? à une deuxième protéine qui est elle-même liée via Z¿1?Z¿2 ? à une protéine Y1 qui peut soit correspondre à Y, soit être différente de Y, et le peptide de transport permettant d'obtenir un meilleur taux de sécrétion de Y et/ou Y¿m?. Selon l'invention: Px est une séquence d'ADN de promoteur choisie de manière à rendre possible l'obtention de rendements optimaux de la protéine d'intérêt; Sx est un ADN quelconque qui en conséquence code pour une séquence signal ou de tête qui permet l'obtention de rendements optimaux; B¿n? correspond à 1-15 acides aminés codables génétiquement ou une liaison chimique; Z est un codon d'un acide aminé