RECOMBINANT TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE WITH IMPROVED FUNCTIONALITY

Truncated terminal deoxynucleotidyl transferase (TdT) derivative from calf thymus characterized in that the derivative in comparison to the native TdT is N- terminally truncated by up to 161 amino acids and has a 20- 30-fold higher enzyme activity in solutions containing Co~ions, and it's recom...

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Hauptverfasser:	SUPPMANN, BERNHARD, SCHMIDT, MANFRED, SOBEK, HARALD, SCHNEIDINGER, BERND, MUELLER, RAINER, SONN, KIRSTEN, PAJATSCH, MARKUS, CURDT, INGO
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Truncated terminal deoxynucleotidyl transferase (TdT) derivative from calf thymus characterized in that the derivative in comparison to the native TdT is N- terminally truncated by up to 161 amino acids and has a 20- 30-fold higher enzyme activity in solutions containing Co~ions, and it's recombinant production and use. Dérivé terminal tronqué de désoxynucléotidyl transférase (TdT) provenant de thymus de veau caractérisé par le fait que, par rapport à la TdT d'origine, jusqu'à 161 acides aminés de la terminaison N du dérivé sont tronqués, et par le fait que l'activité enzymatique du dérivé en solutions contenant des ions Co est 20 à 30 fois plus élevée; le dérivé se distingue aussi par sa production et son utilisation de recombinants.