PROCESSING OF PLASMID-CONTAINING FLUIDS

Disclosed is a method for processing a plasmid-containing fluid comprising contacting the plasmid-containing fluid with two charged membranes and obtaining a plasmid-enriched fluid such as a purified plasmid. In an embodiment, the first charged membrane is positively charged, and the second charged...

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Bibliographische Detailangaben
Hauptverfasser:	NOCHUMSON, SAMUEL, YANG, YUJING, KINSEY, JOE L., JR
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed is a method for processing a plasmid-containing fluid comprising contacting the plasmid-containing fluid with two charged membranes and obtaining a plasmid-enriched fluid such as a purified plasmid. In an embodiment, the first charged membrane is positively charged, and the second charged membranes is positively or negatively charged membrane. In a preferr ed embodiment, both first and second charged membranes are positively charged. In another embodiment, the plasmid-containing fluid is contacted with two positively charged membranes and a third charged membrane. The present invention further provides a system and a kit for processing plasmid- containing fluids.