METABOLICALLY ENGINEERED MICROBIAL CELL WITH AN ALTERED METABOLITE PRODUCTION

The invention relates to a manipulation of the assimilation of a nutrient source in a microbial cell. The nutrient source is preferably ammonia and the uptake thereof in said microbial cell, preferably a yeast cell, is linked to an altered production of a metabolite such as ethanol or glycerol. Micr...

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Bibliographische Detailangaben
Hauptverfasser:	KIELLAND-BRANDT, MORTEN C, NIELSEN, JENS, NISSEN, TORBEN LAUESGAARD
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a manipulation of the assimilation of a nutrient source in a microbial cell. The nutrient source is preferably ammonia and the uptake thereof in said microbial cell, preferably a yeast cell, is linked to an altered production of a metabolite such as ethanol or glycerol. Microbial cells according to the invention comprise a first expressible enzyme activity which, when expressed in said microbial cell, is controlling assimilation in said cell of a nutrient source, and optionally a second expressible enzyme activity which, when expressed in said microbial cell, is controlling assimilation in said cell of a nutrient source, said second expressible enzyme activity being non-identical to said first expressible enzyme activity, and a reduced expression or no expression of a third expressible enzyme activity which, when expressed in said microbial cell, is controlling assimilation in said cell of a nutrient source, said third expressible enzyme activity being non-identical to any and both of said first and second expressible enzyme activities. Cells according to the invention are capable of producing increased amounts of ethanol in a fermentation.