REVERSE TRANSCRIPTION ACTIVITY FROM BACILLUS STEAROTHERMOPHILUS DNA POLYMERASE IN THE PRESENCE OF MAGNESIUM

The present invention is directed to a thermostable DNA polymerase from Bacillus stearothermophilusfor use in reverse transcription and/or reverse transcriptase-polymerase chain reaction (RT-PCR), where said DNA polymerase shows magnesium ion dependent reverse transcriptase activity and in the subst...

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Bibliographische Detailangaben
1. Verfasser: SCHANKE, JUDITH E. T
Format: Patent
Sprache:eng ; fre
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Zusammenfassung:The present invention is directed to a thermostable DNA polymerase from Bacillus stearothermophilusfor use in reverse transcription and/or reverse transcriptase-polymerase chain reaction (RT-PCR), where said DNA polymerase shows magnesium ion dependent reverse transcriptase activity and in the substantial absence of manganese ions.