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The present invention relates to the use of DNA primers homologous to MITE i n a method for detecting polymorphisms of a nucleic acid sequence. The method comprises the steps of amplifying nucleic acid sequences using a first prime r homologous to a MITE in combination with another primer homologous...
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Format: | Patent |
Sprache: | eng ; fre |
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Zusammenfassung: | The present invention relates to the use of DNA primers homologous to MITE i n a method for detecting polymorphisms of a nucleic acid sequence. The method comprises the steps of amplifying nucleic acid sequences using a first prime r homologous to a MITE in combination with another primer homologous or not to a MITE, separating fragments of the nucleic acid sequences amplified, and analyzing the fragments obtained in relation to reference fragments obtained from amplification of a nucleic acid sequence with the primer homologous to MITE for determining polymorphism in the nucleic acid sequence. DNA primers homologous to MITE may also be used in genotyping, fingerprinting, mapping o r cloning method in accordance with the invention. |
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