MAGNETIC DNA EXTRACTION KIT FOR PLANTS

A method and kit for the extraction of DNA from plants is provided, which quickly yields plant DNA with a high level of purity. The method isolates DN A (genomic, chloroplast, and/or mitochondrial DNA) using immobilized anionic groups, preferably on a chromatographic substrate or more preferably mag...

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Bibliographische Detailangaben
Hauptverfasser:	MACCECCHINI, MARIA-LUISA, GORE, MITCHELL T
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method and kit for the extraction of DNA from plants is provided, which quickly yields plant DNA with a high level of purity. The method isolates DN A (genomic, chloroplast, and/or mitochondrial DNA) using immobilized anionic groups, preferably on a chromatographic substrate or more preferably magneti c beads derivatized with anionic groups such as diethylaminoethyl (DEAE) via a n anion-exchange interaction. The purified DNA is then eluted with ions (typically a salt solution). RNA can be removed by digestion with RNAse.