DETECTION OF BIOLOGICAL WARFARE AGENTS

Methods and devices are provided for the detection of bacterial agents such agents as Bacillus anthracis and Clostridium botulinum with high sensitivity and selectivity. More specifically, methods and devices are based on a phosphorescence-emission detection system using chelate-stabilized lantanide...

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Bibliographische Detailangaben
Hauptverfasser:	RAJAN, KRISHNASWAMY S, MAINER, STEPHEN S
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods and devices are provided for the detection of bacterial agents such agents as Bacillus anthracis and Clostridium botulinum with high sensitivity and selectivity. More specifically, methods and devices are based on a phosphorescence-emission detection system using chelate-stabilized lantanide s (e.g, Eu(III), Tb(III), and Sm(III)) to detect various spore-specific small organic molecules (e.g, dipicolinic acid, diaminopimelic acid, n-acetlymuram ic acid, and the like). By careful selection of the chelating agent or ligand coordinated to the lanthanide, both high specificity and selectivity can be obtained. Examples of suitable and preferred sensor systems include N-(2- hydroxyethyl)ethylenediaminetriacetic acid (HEDTA) and N-(2- hydroxyethyl)iminodiacetic acid (HEIDA) combined with europium (III) and/or terbium (III). The chelate-stabilized lanthanides react with the spore- specific "target" molecules to form a characteristically phosphorescent product which can then be detected.