PREPARATION OF PANCREATIC PROCARBOXYPEPTIDASE B, ISOFORMS AND MUTEINS THEREOF AND THEIR USE

PREPARATION OF PANCREATIC PROCARBOXYPEPTIDASE B, ISOFORMS AND MUTEINS THEREOF AND THEIR USE

The invention relates to a process for the preparation of pancreatic carboxypeptidase B or an isoform or a mutein of carboxypeptidase B, where (a) a natural or unnatural precursor form of carboxypeptidase B is expressed in a microorganism by secretion, (b) the precursor form expressed by secretion i...

Ausführliche Beschreibung

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Bibliographische Detailangaben
1. Verfasser: HABERMANN, PAUL
Format: Patent
Sprache:eng ; fre
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to a process for the preparation of pancreatic carboxypeptidase B or an isoform or a mutein of carboxypeptidase B, where (a) a natural or unnatural precursor form of carboxypeptidase B is expressed in a microorganism by secretion, (b) the precursor form expressed by secretion is purified and (c) the purified precursor form is converted into the active carboxypeptidase B, the isoform or the mutein thereof by an enzymatic treatment, a nucleic acid construct, its use in the process mentioned and its preparation, and a host cell, its preparation and use in a process for the preparation of pancreatic carboxypeptidase B or an isoform or a mutein thereof.