CELL-CULTURE AND POLYMER CONSTRUCTS

Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes ma y be grown on dextran microcarrier beadlets and then the beadlets digested usi ng dextranase to separate the chondrocytes from the carrier. Cells can also...

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Bibliographische Detailangaben
Hauptverfasser:	SHIKANI, ALAN H, HUNGERFORD, DAVID S, DOMB, ABRAHAM J, SOHRABI, AFSHIN, FRONDOZA, CARMELITA G
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G BANDAGES, DRESSINGS OR ABSORBENT PADS BEER BIOCHEMISTRY CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS DERIVATIVES THEREOF DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF,TUBULAR STRUCTURES OF THE BODY, E.G. STENTS DISINFECTION, STERILISATION, OR DEODORISATION OF AIR ENZYMOLOGY FILTERS IMPLANTABLE INTO BLOOD VESSELS FIRST-AID KITS FOMENTATION GENERAL PROCESSES OF COMPOUNDING HUMAN NECESSITIES HYGIENE MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES MEDICAL OR VETERINARY SCIENCE METALLURGY METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES POLYSACCHARIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PROSTHESES SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP TREATMENT OR PROTECTION OF EYES OR EARS VINEGAR WINE WORKING-UP
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Beschreibung
Zusammenfassung:	Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes ma y be grown on dextran microcarrier beadlets and then the beadlets digested usi ng dextranase to separate the chondrocytes from the carrier. Cells can also be grown on chitosan microcarriers to be used for implantation. In addition, cells can be grown on polysaccharide polymers to be used as implant devices. Various polymers serve as scaffolds for cells to be used for implantation. T he polymers can be used for cell culture as well as for preparing scaffolds useful for tissue replacement such as cartilage tissue.