IMMOBILISING POLYNUCLEOTIDES

A method of immobilising a nucleotide or polynucleotide on a solid support, comprises: providing the solid support having a functionalised surface of th e formula B - S - S - L where B is the solid support and L is a leaving group; providing a nucleotide or polynucleotide comprising at least one pho...

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Bibliographische Detailangaben
1. Verfasser:	ROSLER, ANGELIKA JOSEFINE
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of immobilising a nucleotide or polynucleotide on a solid support, comprises: providing the solid support having a functionalised surface of th e formula B - S - S - L where B is the solid support and L is a leaving group; providing a nucleotide or polynucleotide comprising at least one phosphorothioate group; and coupling the two together by means of a sulphide exchange reaction. The product has the structure B - S - S - P(O)R1R2, where - POR1R2 represents a nucleotide or a polynucleotide. Arrays of the immobilise d polynucleotides are useful for performing APEX or SBH assays.