METHODS OF PURIFYING RECOMBINANT HUMAN ERYTHROPOIETIN FROM CELL CULTURE SUPERNATANTS

The present invention relates, in general, to a method of purifying recombinant human erythropoietin (EPO). The present invention also relates to a substantially pure EPO. The method comprises a differential precipitation, an hydrophobic interaction chromatography, various concentration and diafiltr...

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Bibliographische Detailangaben
Hauptverfasser:	CRISCUOLO, MARCELO, VIDAL, JUAN ALEJANDRO, CARCAGNO, CARLOS MIGUEL, MELO, CARLOS
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	CHEMISTRY METALLURGY ORGANIC CHEMISTRY PEPTIDES
Online-Zugang:	Volltext bestellen
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Beschreibung
Zusammenfassung:	The present invention relates, in general, to a method of purifying recombinant human erythropoietin (EPO). The present invention also relates to a substantially pure EPO. The method comprises a differential precipitation, an hydrophobic interaction chromatography, various concentration and diafiltration steps, tandem anionic and cationic exchange chromatographies and molecular exclusion chromatography for the obtaining of pure EPO. The method does not comprise high performance liquid chromatography steps. The invention also comprises the EPO obtained according to the claimed procedure.