INCREASING STEARATE CONTENT IN SOYBEAN OIL BY EXPRESSION OF ACYL-ACP THIOSTERASE

This invention relates to a method for increasing stearate as a component of total triglycerides found in soybean seed. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5' to 3' direction of transcription, a promote...

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Bibliographische Detailangaben
1. Verfasser:	KRIDL, JEAN C
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES ANIMAL HUSBANDRY BAKERY PRODUCTS BAKING BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE DETERGENTS EDIBLE DOUGHS EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKINGOILS ENZYMOLOGY ESSENTIAL OILS FATTY ACIDS THEREFROM FISHING FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J FORESTRY HUMAN NECESSITIES HUNTING MAKING THEREOF METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MILK OR CHEESE SUBSTITUTES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PERFUMES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PRESERVATION THEREOF PROCESSES USING MICROORGANISMS PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES TRAPPING TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to a method for increasing stearate as a component of total triglycerides found in soybean seed. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5' to 3' direction of transcription, a promoter functional in a soybean plant seed cell, a DNA sequence encoding an acyl-ACP thioesterase protein having substantial activity on C18:0 acyl-ACP substrates, and a transcription termination region functional in a plant cell. The present invention also provides a soybean seed with about 33 weight percent or greater stearate as a component of total fatty acids found in seed triglycerides. Cette invention concerne un procédé servant à augmenter le stéarate en tant que composant de l'ensemble des triglycérides présents dans la semence de soja. De manière générale, le procédé consiste à faire pousser un plant de soja qui a un gène hybride d'ADN intégré dans son génome. Ledit gène comporte, dans la direction 5' vers 3' de la transcription, un agent promoteur opérationnel dans une semence de plant de soja, une séquence d'ADN codant une protéine thioestérasique d'acyle ACP ayant une activité importante sur les substrats d'acyle ACP C18:0, et une zone de terminaison de transcription opérationnelle dans une cellule de la plante. Cette invention concerne également une semence de soja possédant un stéarate d'environ 33 % en poids ou un stéarate plus important en tant que composant de l'ensemble des acides gras présents dans les triglycérides des semences.