SALICYLIC ACID PATHWAY GENES AND THEIR USE FOR THE INDUCTIONOF RESISTANCE IN PLANTS

This invention describes a method to induce pathogen resistance in plants, characterized in that plants are transformed with an expression cassette harboring a gene coding for an isochorismate synthase. More specifically, th is method is characterized in that the gene coding for isochorismate syntha...

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Hauptverfasser: CROES, ANTON FELIX, VERBERNE, MARIA CATHARINA, MELCHERS, LEO SJOERD, BOL, JOHN FERDINAND, SIMONS, LAMBERTUS HENRICUS, VERPOORTE, ROBERT, LINTHORST, HUBERTUS JOSEPHUS MARIA, WULLEMS, GEORGE JOSEPH, STUIVER, MAARTEN HENDRIK, MORENO, PAULO R. H, CUSTERS, JEROME HUBERTINA HENRICUS VICTOR, VAN TEGELEN, LEONARDUS JOHANNES PETRONELLA
Format: Patent
Sprache:eng ; fre
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Zusammenfassung:This invention describes a method to induce pathogen resistance in plants, characterized in that plants are transformed with an expression cassette harboring a gene coding for an isochorismate synthase. More specifically, th is method is characterized in that the gene coding for isochorismate synthase i s selected from a group consisting of entC, orfA, pchA and ICS, this last gene preferably the ICS gene from Catharantus roseus. Another embodiment of the invention is a method according to the method described above, characterized in that plants are additionally transformed with an expression cassette harboring a gene coding for an isochorismate pyruvate lyase, preferably on t he same expression cassette as the gene coding for isochorismate synthase. The gene coding for isochorismate pyruvate lyase is preferably selected from the group consisting of orfD and pchB. A further aspect of the invention is a protein having isochorismate synthase activity which is isolated from Catharantus roseus. Still a further aspect of the invention is the nucleotid e sequence comprising the 5' regulatory region which is naturally found to regulate the expression of the ICS gene in Catharantus roseus.