METHOD FOR DETECTION OF TARGET NUCLEIC ACIDS USING PCR

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising (a) subjecting said sample to an amplification reaction using a set of nucleotides, at least one of whi ch is fluorescently labelled, (b) contacting amplification product with a probe under cond...

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Bibliographische Detailangaben
Hauptverfasser:	LEE, MARTIN ALAN, LESLIE, DARIO LYALL
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising (a) subjecting said sample to an amplification reaction using a set of nucleotides, at least one of whi ch is fluorescently labelled, (b) contacting amplification product with a probe under conditions in which the probe will hybridise to said targ et sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and (c) monitoring fluorescence of said sample. The method can be used to quantitate the amount of target nucleic acid in the sample as well as to determine sequence characteristics. Kits for effecting the method are also claimed.