ELECTROPHORETIC SEPARATION OF NUCLEIC ACIDS FROM PROTEINS AT LOW PH

The present invention relates to methods for separating nucleic acids from other cellular debris, especially substances that carry a net positive charge at low pH, by electrophoresis under acid conditions. In the purification method of the present invention, nucleic acids are separated from proteins...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: BACKUS, JOHN WESLEY, KREADER, CAROL ANN
Format: Patent
Sprache:eng ; fre
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Beschreibung
Zusammenfassung:The present invention relates to methods for separating nucleic acids from other cellular debris, especially substances that carry a net positive charge at low pH, by electrophoresis under acid conditions. In the purification method of the present invention, nucleic acids are separated from proteins found in the same biological sample by applying the sample to an electrophesis gel and subjecting the sample to electrophoresis under acid conditions to separate the nucleic acids from the proteins. The optimum pH may differ for different sample types but can be readily determined by those skilled in the art. Preferably, the separation is performed at a pH of about 2 to about 4. More preferably, electrophoresis is carried out at a pH of 2.5