BACILLUS THURINGIENSIS CRYET33 AND CRYET34 COMPOSITIONS AND USES THEREFOR

Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal tox...

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Bibliographische Detailangaben
Hauptverfasser:	DONOVAN, JUDITH C, SLANEY, ANNETTE C, DONOVAN, WILLIAM P
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FORESTRY HUMAN NECESSITIES HUNTING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PEST REPELLANTS OR ATTRACTANTS PHYSICS PLANT GROWTH REGULATORS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TESTING TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode the coleopteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention. L'invention concerne des souches de Bacillus thuringiensis comprenant de nouvelles protéines cristallines présentant une activité insecticide contre les coléoptères, notamment contre le tribolium rouge de la farine (Tribolium castaneum) à l'état larvaire et contre le scarabée japonais (Popillia japonica) à l'état larvaire. On décrit aussi de nouveaux gènes de toxines cristallins du B. thuringiensis, appelés cryET33 et cryET34, qui codent pour les protéines cristallines à pouvoir toxique contre les coléoptères; le gène cryET34 codant pour la protéine cristalline CryET33 (29-kDa), et le gène cryET34, pour la protéine cristalline 14-kDa CryET34. Les protéines cristallines CryET33 et CryET34 ont un pouvoir toxique contre le tribolium rouge de la farine à l'état larvaire et contre le scarabée japonais à l'état larvaire. L'invention concerne également des procédés de fabrication et d'utilisation de cellules transgéniques comprenant les nouvelles séquences d'acide nucléique faisant l'objet de l'invention.