METHODS FOR TARGETING, ENRICHING, DETECTING AND/OR ISOLATING TARGET NUCLEIC ACID SEQUENCE USING RECA-LIKE RECOMBINASE

A method for targeting, enriching, detecting and isolating a double-stranded nucleic acid target sequence by adding heterologous probes to the reaction system along with the reduced amount of homologous probes in the process of homologous pairing and/or strand exchange between the homologous probe a...

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Bibliographische Detailangaben
Hauptverfasser: MUKAI, ELI, KUSUMI, KAYO, OBATA, KAZUAKI, KIGAWA, KOJI, YAMANAKA, MIKAYO
Format: Patent
Sprache:eng ; fre
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Zusammenfassung:A method for targeting, enriching, detecting and isolating a double-stranded nucleic acid target sequence by adding heterologous probes to the reaction system along with the reduced amount of homologous probes in the process of homologous pairing and/or strand exchange between the homologous probe and the target sequence in the presence of a RecA-like recombinase.