IMMOBILIZED ENZYME AND ITS USE FOR THE PROCESSING OF TRIGLYCERIDE OILS

Process for the immobilization of an enzyme, characterized by the steps comprising: a) selecting an amphiphilic enzyme for immobilization, b) preparing an emulsion comprising a continuous hydrophobic phase and a dispersed aqueous phase in which are dissolved the enzyme and material suitable to act a...

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Bibliographische Detailangaben
Hauptverfasser:	VAN PUTTE, KAREL P. A. M, GEURTSEN, JOHAN PAUL T, GROTE, MARTIN ROGER
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Process for the immobilization of an enzyme, characterized by the steps comprising: a) selecting an amphiphilic enzyme for immobilization, b) preparing an emulsion comprising a continuous hydrophobic phase and a dispersed aqueous phase in which are dissolved the enzyme and material suitable to act as carrier for the enzyme when the next step is carried out, c) removing water from the aqueous phase until this phase turns into solid enzyme coated particles. A preferred aqueous phase is prepared with a crude lipase fermentation liquid from which the biomass has been removed and which still contains fermentation residues suitable to act as carrier. Processes using the immobilized enzyme comprise interesterification, deacidification and degumming of triglyceride oils and show a very low enzyme consumption. Besides lipases suitable amphiphilic enzymes are phospholipases.