METHOD FOR PURIFYING POLYPEPTIDES
A process is provided for selectively separating a polypeptide of interest f rom components of differing in a mixture comprising the steps of: a) passing the mixture through underivatized silica particles such that the polypeptide adheres to the sili ca particles; b) washing the silica particles to...
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| Format: | Patent |
| Sprache: | eng ; fre |
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| Zusammenfassung: | A process is provided for selectively separating a polypeptide of interest f rom components of differing in a mixture comprising the steps of: a) passing the mixture through underivatized silica particles such that the polypeptide adheres to the sili ca particles; b) washing the silica particles to remove impurities; and c) eluting the pol ypeptide from the silica particles with a buffer compris ing an alcoholic or polar aprotic solvent and an alkaline earth, an alkali metal, o r an inorganic ammonium salt, |
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