COAMPLIFICATION OF TARGET NUCLEIC ACIDS USING VOLUME EXCLUSION AGENT, IN REACTION COMPOSITION, TEST KIT AND TEST DEVICE USEFUL THEREFOR

Multiple target nucleic acids are amplified using polymerase chain reaction in the presence of a nonionic, polymeric volume exclusion agent. The amplification efficiency of low copy target nucleic acids is increased in the presence of the volume exclusion agent even though reduced primer levels are...

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Bibliographische Detailangaben
1. Verfasser:	BACKUS, JOHN WESLEY
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Multiple target nucleic acids are amplified using polymerase chain reaction in the presence of a nonionic, polymeric volume exclusion agent. The amplification efficiency of low copy target nucleic acids is increased in the presence of the volume exclusion agent even though reduced primer levels are used. In this manner, amplification efficiency of a given target nucleic acid can be more readily manipulated.