ASSAY METHODS AND COMPOSITIONS FOR DETECTING AND EVALUATING THE INTRACELLULAR TRANSDUCTION OF AN EXTRACELLULAR SIGNAL

Transcription based assays that identify extracellular signals that modulate the activity of cell surface proteins are provided. Extracellular signals are indentified by measuring the amount of transcription o f a reporter gene in a recombinant cell that expresses the cell surface protein and contai...

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Bibliographische Detailangaben
Hauptverfasser:	HARPOLD, MICHAEL MILLER, BRUST, PAUL
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Zusammenfassung:	Transcription based assays that identify extracellular signals that modulate the activity of cell surface proteins are provided. Extracellular signals are indentified by measuring the amount of transcription o f a reporter gene in a recombinant cell that expresses the cell surface protein and contains DNA encoding the reporter gene u nder the transcriptional control of a promoter that is regulated, directly or indirectly, by the cell surface protein. The assa ys provide a means for identifying potiential pharmaceutical compounds that can be used to treat disease by virtue of their agonistic or anta gonistic effects on the cell surface protein. Recombinant cells that express cell surface receptors and that contain reporter gene constructs that include transcriptional regulatory elements that are responsive to the activity of the cell surface receptors are a lso provided.