SPECIES-SPECIFIC METHOD FOR IDENTIFYING INFECTIVITY OF EIMERIA SPECIES

JLT 16Y 18374Y TITLE OF THE INVENTION SPECIES-SPECIFIC METHOD FOR IDENTIFYING INFECTIVITY OF ETMERIA SPECIES Unique methods are disclosed to determine the relative infectivity of each of multiple species of Eimeria in a multivalent live vaccine. Genomic deoxyribonucleic acid (DNA) is extracted from...

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Hauptverfasser:	LIBERATOR, PAUL A, ELBRECHT, ALEX, PROFOUS-JUCHELKA, HELEN, FEIGHNER, SCOTT D, PERKINS-BARROW, ANN, ANDERSON, JENNIFER W, CHAKRABORTY, PRASANTA R, DASHKEVICZ, MICHAEL
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	JLT 16Y 18374Y TITLE OF THE INVENTION SPECIES-SPECIFIC METHOD FOR IDENTIFYING INFECTIVITY OF ETMERIA SPECIES Unique methods are disclosed to determine the relative infectivity of each of multiple species of Eimeria in a multivalent live vaccine. Genomic deoxyribonucleic acid (DNA) is extracted from intestinal epithelia and mucosa of vaccinated birds and amplified using the polymerase chain reaction (PCR). The reaction allows efficient amplification of fragments from every small subunit ribosomal RNA (ssrRNA) gene in the target pool. Unique species-specific hybridization probes complimentary to sequences within the heterogeneous pool of PCR products are used to quantitate the relative levels of each of the Eimeria species in the infected hosts. The same unique probes are used to identify and quantitate species specific ssrRNA sequences in total RNA extracted from intestinal epithelia and mucosa of vaccinated chickens. The same unique probes are also used to identify and quantitate species specific ssrRNA sequences in genomic DNA isolated from mixed oocyst population purified from decal material obtained from vaccinated chickens.