NON-SPECIFIC DNA AMPLIFICATION

2067363 9105861 PCTABS00005 A method of non-specifically amplifying different-sequence fragments in a mixture of duplex DNA fragments is disclosed. The fragments are provided with end linkers, and the mixture is amplified by successive primer-initiated replication. Also disclosed is a method of clon...

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Hauptverfasser: WEISSMAN, SHERMAN M, REYES, GREGORY R, JORGENSON, LINDA M, LOVETT, MICHAEL
Format: Patent
Sprache:eng ; fre
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Zusammenfassung:2067363 9105861 PCTABS00005 A method of non-specifically amplifying different-sequence fragments in a mixture of duplex DNA fragments is disclosed. The fragments are provided with end linkers, and the mixture is amplified by successive primer-initiated replication. Also disclosed is a method of cloning cDNA species which are homologous to a region of contiguous genomic DNA and are selected from a mixture of cDNA species.