CLONING AND EXPRESSION OF XYLANASE GENES FROM FUNGAL ORIGIN

ABSTRACT OF THE DISCLOSURE Methods and expression constructs are provided for the cloning of genes from fungal origin which encode polypeptides having xylanase activity and their overexpression in a selected microbial host cell. Xylanases of fungal origin generally have lower pH optima and remain st...

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Hauptverfasser:	VISSER, JACOB, HILLE, JAN D. R, VAN OOYEN, ALBERT J. J, VAN DEN BROECK, HENRIETTE C, HARDER, ABRAHAM, DE GRAAFF, LEENDERT H
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	APPARATUS THEREFOR BAKERY PRODUCTS BAKING BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA EDIBLE DOUGHS ENZYMOLOGY FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PAPER-MAKING PRESERVATION THEREOF PROCESSES USING MICROORGANISMS PRODUCTION OF CELLULOSE PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REGENERATION OF PULPING LIQUORS SPIRITS TEXTILES THEIR TREATMENT, NOT COVERED BY OTHER CLASSES TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS VINEGAR WINE
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Zusammenfassung:	ABSTRACT OF THE DISCLOSURE Methods and expression constructs are provided for the cloning of genes from fungal origin which encode polypeptides having xylanase activity and their overexpression in a selected microbial host cell. Xylanases of fungal origin generally have lower pH optima and remain stable over a broader pH range than do xylanases of bacterial origin. The present invention provides for the high level production of fungal xylanases which may be used in a variety of industrial applications requiring xylanase activity at a low pH.