ISOLATION AND PURIFICATION OF LUNG SURFACTANT PROTEIN

Methods are provided for the isolation and purification of recombinant lung surfactant protein. The methods involve a multistep procedure including extraction with a C1-C4 aliphatic alcohol and chromatographic purification steps which employ a C1-C4 aliphatic alcohol as eluant. Purified proteins obt...

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Hauptverfasser:	BUCKLEY, DOUGLAS, BENSON, BRADLEY J, LESIKAR, DAVID, NAIDU, ASHA, SILVERNESS, KATIE B
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	Methods are provided for the isolation and purification of recombinant lung surfactant protein. The methods involve a multistep procedure including extraction with a C1-C4 aliphatic alcohol and chromatographic purification steps which employ a C1-C4 aliphatic alcohol as eluant. Purified proteins obtained using the disclosed isolation and purification steps are pro- vided as well. 2065323 9200993 PCTABS00010 Methods are provided for the isolation and purification of recombinant lung surfactant protein. The methods involve a multistep procedure including extraction with a C1-C4 aliphatic alcohol and chromatographic purification steps which employ a C1-C4 aliphatic alcohol as eluant. Purified proteins obtained using the disclosed isolation and purification steps are provided as well.