USE OF AUTOLOGOUS PROMOTERS TO EXPRESS GENE PRODUCTS IN BORDETELLA

Autologous promoters are used to effect expression of gene products in pertussis strains. Hybrid pertussis genes are constructed comprising a structural pertussis gene fused at an ATG codon to a native but autologous pertussis promoter. B. pertussis, transformed by the hybrid gene by insertion into...

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Bibliographische Detailangaben
Hauptverfasser:	LOOSMORE, SHEENA, ZEALEY, GAVIN, YACOOB, REZA K, KLEIN, MICHEL
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Autologous promoters are used to effect expression of gene products in pertussis strains. Hybrid pertussis genes are constructed comprising a structural pertussis gene fused at an ATG codon to a native but autologous pertussis promoter. B. pertussis, transformed by the hybrid gene by insertion into the chromosome of the organism by homologous recombination at specific loci, effects expression of the antigenic protein for which the structural gene codes at a production rate different from that achieved for the homologous gene. Specific strains and plasmids are descibed. The technique is applicable to a wide variety of gene products and organisms.