MUTANT MICROBIAL .ALPHA.-AMYLASES WITH INCREASED THERMAL, ACID, AND/OR ALKALINE STABILITY

Thermostable and acid stable .alpha.-amylases are provided as expression products of genetically engineered .alpha.-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphate method and enzymatic m...

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Hauptverfasser: LAROCHE, YVES, VOLLEBREGT, ADRIANUS W. H, STANSSENS, PATRICK, QUAX, WILHELMUS J, LAUWEREYS, MARC
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creator LAROCHE, YVES
VOLLEBREGT, ADRIANUS W. H
STANSSENS, PATRICK
QUAX, WILHELMUS J
LAUWEREYS, MARC
description Thermostable and acid stable .alpha.-amylases are provided as expression products of genetically engineered .alpha.-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphate method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant .alpha.-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.
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Both chemical and enzymatic mutagenesis methods are e.g. the bisulphate method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant .alpha.-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.</abstract><edition>5</edition><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
BLEACHING LEATHER OR FURS
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
LAUNDERING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TEXTILES
TREATMENT OF TEXTILES OR THE LIKE
TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS,FABRICS, FEATHERS, OR FIBROUS GOODS MADE FROM SUCH MATERIALS
VINEGAR
WINE
title MUTANT MICROBIAL .ALPHA.-AMYLASES WITH INCREASED THERMAL, ACID, AND/OR ALKALINE STABILITY
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