MUTANT MICROBIAL .ALPHA.-AMYLASES WITH INCREASED THERMAL, ACID, AND/OR ALKALINE STABILITY

Thermostable and acid stable .alpha.-amylases are provided as expression products of genetically engineered .alpha.-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphate method and enzymatic m...

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Bibliographische Detailangaben
Hauptverfasser:	LAROCHE, YVES, VOLLEBREGT, ADRIANUS W. H, STANSSENS, PATRICK, QUAX, WILHELMUS J, LAUWEREYS, MARC
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY BLEACHING LEATHER OR FURS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR LAUNDERING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TEXTILES TREATMENT OF TEXTILES OR THE LIKE TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS,FABRICS, FEATHERS, OR FIBROUS GOODS MADE FROM SUCH MATERIALS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Thermostable and acid stable .alpha.-amylases are provided as expression products of genetically engineered .alpha.-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphate method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant .alpha.-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.