PROCESS FOR PREPARING AND PURIFYING ALPHA-INTERFERON

This invention relates to a process for the preparation of recombinant interferon wherein the host organism containing the interferon gene is cultivated, the cells are harvested and disrupted, the cell debris is removed, the interferon is concentrated and subjected to preliminary purification by tan...

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Bibliographische Detailangaben
Hauptverfasser:	BODO, GERHARD, FALKNER, EDGAR, LINDNER, SILVIA JUTTA, MAURER-FOGY, INGRID
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to a process for the preparation of recombinant interferon wherein the host organism containing the interferon gene is cultivated, the cells are harvested and disrupted, the cell debris is removed, the interferon is concentrated and subjected to preliminary purification by tandem chromatography, the eluate is adjusted to pH 4.0-4.8 to remove impurities, the interferon is finally purified by chromatography on a cation exchanger column with a volatile buffer as eluant, and is then lyophilised whereby a highly pure, non-immunogenic, homogeneous .alpha.-interferon having anti-viral and immunoregulatory activities is obtained, to the proteins produced by the process and to the use thereof.