BAR1 SECRETION SIGNAL

The invention provides a DNA construct capable of being transformed into a yeast or mammalian cell for the secretion of heterologous proteins therefrom. The construct comprises a transcriptional promoter operably linked to a DNA sequence encoding a signal peptide, followed in reading frame by a seco...

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Bibliographische Detailangaben
Hauptverfasser:	WELCH, SUSAN K, MACKAY, VIVIAN L
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a DNA construct capable of being transformed into a yeast or mammalian cell for the secretion of heterologous proteins therefrom. The construct comprises a transcriptional promoter operably linked to a DNA sequence encoding a signal peptide, followed in reading frame by a second DNA sequenca encoding a portion of the BAR1 gene product, including at least a portion of the C-terminal domain and a heterologous protein or polypeptide. The invention may be used to express a variety of proteins including urokinase, insulin, platelst-derived growth factor and analogs thereof. The signal sequence and the sequence encoding a portion of the Barrier Cterminal region encode a hybrid secretory peptide which is then used to direct the secretion of heterologous proteins or polypeptides from host cells. The secretory peptide is subsequently cleaved from the heterologous protein.