RECOMBINANT DNA EXPRESSION VECTORS AND METHOD FOR GENE EXPRESSION

The present invention provides a process for preparing selectable and autonomously replicating recombinant DNA expression vectors which comprise 1) a transcriptional and translational activating sequence which is in the reading frame of a nucleotide sequence that codes for a peptide or polypeptide,...

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Bibliographische Detailangaben
Hauptverfasser:	SCHONER, BRIGITTE E, SCHONER, RONALD G
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a process for preparing selectable and autonomously replicating recombinant DNA expression vectors which comprise 1) a transcriptional and translational activating sequence which is in the reading frame of a nucleotide sequence that codes for a peptide or polypeptide, 2) a translational stop signal; 3) a translational start signal which is in the reading frame of a nucleotide sequence that codes for a functional polypeptide; and 4) an additional translational stop signal. The above-described nucleotide sequences are engineered to generate, upon transcription, a translatable polycistronic mRNA. The invention further provides a method of use.